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目的:建立测定木豆叶中牡荆苷含量的HPLC方法,研究不同产地及品种木豆叶中牡荆苷的含量差异。方法:采用HPLC法测定木豆叶中牡荆苷的含量。色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);流动相为甲醇-1%冰醋酸(29:71);检测波长:330 nm;流速:1.0 mL/min;进样量:10μL;柱温:40℃。结果:牡荆苷在0.0016～1.01μg范围内具有良好的线性关系,r=0.9999;平均回收率为96.523%,RSD为1.85%。结论:本方法简便、准确、重现性好,可用于木豆叶中牡荆苷含量的测定。不同产地及品种木豆叶中牡荆苷含量差异较大。
OBJECTIVE: To establish a HPLC method for the determination of vitexin in Gynura divaricata, and to study the content differences of Vitexin in Gynura divaricata from different habitats and varieties. Methods: HPLC method was used to determine the contents of vitexin in The column was Diamonsil C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol-1% glacial acetic acid (29:71). The detection wavelength was 330 nm. The flow rate was 1.0 mL / min. The injection volume was 10 μL. Temperature: 40 ℃. Results: Vitexin had a good linear relationship in the range of 0.0016 ~ 1.01 μg with r = 0.9999; the average recovery was 96.523% and the RSD was 1.85%. Conclusion: The method is simple, accurate and reproducible. It can be used to determine the content of vitexin in the leaves of. The contents of vitexin in the leaves of different varieties and varieties were different.