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目的研究血管紧张素Ⅱ(AngⅡ)对人肝癌HepG2细胞血管内皮生长因子(VEGF)mRNA表达的影响。方法体外培养人肝癌HepG2细胞,采用不同浓度的AngⅡ进行处理,收集处理后不同时点的细胞,采用逆转录-聚合酶链反应(RT-PCR)半定量法,分别检测AngⅡ处理前后HepG2细胞VEGFmRNA的表达;同时利用MTT法检测AngⅡ对HepG2细胞增殖的影响。结果AngⅡ能刺激HepG2细胞增殖,并增强VEGFmRNA的表达(P<0.05)。这种作用呈浓度-时间依赖效应,当AngⅡ浓度为10-7mol/L,时间为60h时,这种作用达到最高值,且此作用可被AngⅡ1型受体(AT1R)拮抗剂所阻断。结论AngⅡ可以通过AT1R诱导人肝癌细胞VEGFmRNA的表达,对肝癌的生长及转移可能起到一定的促进作用。
Objective To investigate the effect of Ang Ⅱ on the expression of vascular endothelial growth factor (VEGF) mRNA in human hepatoma HepG2 cells. Methods HepG2 cells were cultured in vitro and treated with different concentrations of Ang Ⅱ. The cells were collected at different time points. The expression of VEGF mRNA in HepG2 cells was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) Meanwhile, MTT assay was used to detect the effect of AngⅡ on the proliferation of HepG2 cells. Results Ang Ⅱ stimulated the proliferation of HepG2 cells and enhanced the expression of VEGF mRNA (P <0.05). The effect was concentration-time-dependent. When the concentration of AngⅡ was 10-7mol / L and time was 60h, the effect reached the highest value, and this effect could be blocked by AngⅡ1 receptor (AT1R) antagonist. Conclusion AngⅡ can induce the expression of VEGFmRNA in human hepatocellular carcinoma cells by AT1R, which may promote the growth and metastasis of hepatocellular carcinoma.