枯、黄萎病是世界棉花生产中的两大重要病害。传统育种缺乏抗源,几丁质酶和β-1,3-葡聚糖酶是植物防御体系中的两种防卫因子,两者之间存在协同增效作用。据此构建了4个单价和2个双价基因(分别定位于细胞内或细胞外)的植物表达载体,通过花粉管通道法转化棉花,经PCR和Southern杂交检测以及1996～2000年温室及病圃多代筛选鉴定,已培育出对枯、黄萎病抗性提高的转基因棉花株系。将抗病基因导入国产抗虫棉品种GK19中,还获得了兼抗病、虫的转基因优系。 Withered, Verticillium wilt is the world’s two major diseases in cotton production. The lack of resistance to traditional breeding, chitinase and β-1,3-glucanase are two defense factors in the plant defense system, there is synergy between the two. Four plant expression vectors containing two monovalent and two bivalent genes (located inside or outside the cell respectively) were constructed and transformed into cotton plants by pollen tube pathway. PCR and Southern blotting were used to detect the plant expression in greenhouse and disease For many generations of selection and screening, transgenic cotton lines with improved resistance to withered and Verticillium wilt have been developed. The resistant gene was introduced into the domestic insect-resistant cotton variety GK19, and also obtained the transgenic lines with disease resistance and insect resistance.