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灵芝是木腐型药用真菌。选取分离自海南尖峰岭的3个灵芝菌株Ganoderma sp.Jfl1、Ganodermasp.Jfl3、Ganoderma sp.Jfl5和五指山地区的1个菌株Ganodermalucidum Hz5,利用梯度实验和比较的方法研究不同C源、N源及pH值对灵芝生长的影响,并测定灵芝菌丝体多糖的含量。结果表明,培养基pH值为5~7时适宜灵芝真菌的生长;4个菌株在对C源的利用上无特别的偏好性,均能高效利用醇类和多糖作C源;但对N源的利用具有较明显的规律性,在5种N源培养基上,灵芝菌丝的生长速度依次为:酵母提取物>蛋白胨>酪蛋白>硝酸钾>NaNO2,并且NaNO2能抑制灵芝菌丝的生长。灵芝真菌具有较强分解有机物的能力,对醇类和糖类化合物作为C源均能吸收利用,而有机N源比无机N源更利于灵芝的吸收,人工栽培灵芝其菌丝体内多糖含量减少。
Ganoderma lucidum is a rotten medicinal fungus. Ganoderma sp. Jfl1, Ganodermasp.Jfl3, Ganoderma sp.Jfl5 and Ganoderma lucidum Hz5 were isolated from three Ganoderma lucidum strains collected from Jianfengling in Hainan Province. Gradient experiments and comparative methods were used to study the effects of different C sources, N sources and pH Value on the growth of Ganoderma lucidum, and determination of Ganoderma lucidum polysaccharide content. The results showed that the growth of Ganoderma lucidum fungi was suitable when the medium pH was 5 ~ 7. There was no particular preference for the utilization of C sources in the four strains. Both of the four strains could efficiently utilize alcohols and polysaccharides as C source. However, The growth rate of Ganoderma lucidum mycelia was: yeast extract> peptone> casein> potassium nitrate> NaNO2, and NaNO2 could inhibit the growth of Ganoderma lucidum mycelia . Ganoderma lucidum fungi have a strong ability to decompose organic compounds, alcohols and carbohydrates as a C source can be absorbed, and organic N source than inorganic N source is more conducive to the absorption of Ganoderma lucidum, Ganoderma lucidum artificial cultivation of its mycelium polysaccharide content decreased.