论文部分内容阅读
Purpose:To investigate the influence of the same mechanical loading on osteogenesis and osteoclastogenesis in vitro.Methods:Primary osteoblasts,bone marrow-derived mesenchymal stem cells (BMSCs,cultured in osteoinductive medium) and RAW264.7 cells cultured in osteoclast inductive medium were all subjected to a 1000 μstrain (μs) at 1 Hz cyclic mechanical stretch for 30 min (twice a day).Results:After mechanical stimulation,the alkaline phosphatase (ALP) activity,osteocalcin protein level of the osteoblasts and BMSCs were all enhanced,and the mRNA levels of ALP and collagen type Ⅰ increased.Additionally,extracellular-deposited calcium of both osteoblasts and BMSCs increased.At the same time,the activity of secreted tartrate-resistant acid phosphatase,the number of tartrate-resistant acid phosphatase-positive multinucleated cells,matrix metalloproteinase-9 protein levels of RAW264.7 cells and the extracellular calcium solvency all decreased.Conclusion:The results demonstrated that 1000 μs cyclic mechanical loading enhanced osteoblasts activity,promoted osteoblastic differentiation of BMSCs and restrained osteoclastogenesis of RAW264.7 cells in vitro.