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目的用real-time PCR和RT-PCR检测FP248 mRNA表达,研究其在白血病和肿瘤诊断中的意义。方法用real-time PCR和RT-PCR检测肿瘤细胞株(SGC-7901、A549、95D、SW480、U937、PC3)和白血病(ALL、CML、AML)患者骨髓或外周血以及胃癌等消化道肿瘤患者癌组织中FP248 mRNA的表达水平;用RT-PCR技术检测消化道肿瘤患者外周血单个核细胞(PB-MC)FP248 mRNA的表达。结果SGC-7901细胞株FP248 mRNA强阳性,A549和95D细胞弱阳性,其他细胞株阴性;ALL、CML、AML患者骨髓及PBMC FP248表达上调;胃癌、直肠癌、结肠癌患者癌组织中FP248 mRNA的表达高于癌旁组织,食道癌组织为阴性;胃癌、结肠癌和直肠癌患者PBMC未检测到FP248 mRNA。结论FP 248的表达可能在白血病和消化道肿瘤的发生发展中起重要作用;检测该基因的表达可能有助于白血病和消化道肿瘤的诊断。
Objective To detect the expression of FP248 mRNA by real-time PCR and RT-PCR and to study its significance in the diagnosis of leukemia and tumor. Methods Real-time PCR and RT-PCR were used to detect bone marrow or peripheral blood of patients with tumor cell lines (SGC-7901, A549, 95D, SW480, U937, PC3) and leukemia (ALL, CML, AML) The expression of FP248 mRNA was detected by RT-PCR in peripheral blood mononuclear cells (PB-MC) of patients with gastrointestinal cancer. Results FP248 mRNA was strongly positive in SGC-7901 cells, and weakly positive in A549 and 95D cells, but negative in other cell lines. The expression of FP248 mRNA in bone marrow and PBMC of ALL, CML and AML patients was up-regulated. FP248 mRNA in gastric cancer, rectal cancer and colon cancer The expression of FP248 mRNA in PBMC of gastric cancer, colon cancer and rectal cancer was not detected. Conclusion The expression of FP 248 may play an important role in the development of leukemia and gastrointestinal tumors. Detecting the expression of FP 248 may be helpful for the diagnosis of leukemia and gastrointestinal tumors.