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目的:研究ApoAI对原代培养人Müller细胞生长的影响。方法:原代培养人的视网膜Müller细胞后用不同浓度的ApoAI刺激Müller细胞24 h,采用流式细胞术检测ApoAI作用24 h后Müller细胞的细胞周期,比较各组细胞的细胞周期;采用流式细胞术和荧光照相法检测各组Müller细胞细胞凋亡水平,采用单因素方差分析方法比较各组间不同细胞周期间不同细胞数的变化以及各组细胞的凋亡数。结果与结论:流式细胞术结果显示,ApoAI可以抑制人Müller细胞的增殖,且随ApoAI浓度的增加抑制作用增强(P<0.05);ApoAI对细胞的凋亡无明显作用(P>0.05)。
Objective: To study the effect of ApoAI on the growth of human Müller cells in primary culture. Methods: Müller cells were cultured in primary culture of human retinal Müller cells with different concentrations of ApoAI for 24 h. Flow cytometry was used to detect the cell cycle of Müller cells after 24 h of ApoAI treatment. The cell cycle of each group was compared. The apoptosis of Müller cells in each group was detected by cytometry and fluorescence method. The changes of different cells in different cell cycle and the number of apoptosis in each group were compared by one-way analysis of variance. RESULTS AND CONCLUSION: The results of flow cytometry showed that ApoAI could inhibit the proliferation of human Müller cells, and the inhibitory effect was enhanced with the increase of ApoAI concentration (P <0.05). ApoAI had no significant effect on cell apoptosis (P> 0.05).