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幽门螺杆菌(Helicobacter Pylori,HP)与慢性胃炎、消化性溃疡等胃部疾患密切相关,研究菌种保存具有重要的意义。为此,我们从89年开始研究了 HP 菌种在-70℃条件下,用新鲜脱纤维绵羊全血保存可全部存活14个月,并可保持典型的细菌形态和原有的生化反应特性。材料与方法1.菌株:10株 HP 均从各型胃病患者胃镜活检胃粘膜标本分离培养获得,经传数代稳定后,用作菌种保存。2.培养基:试验中用改良 Campy-Bap(布氏琼脂),按有关文献报道配制。3.保藏液:为新鲜脱纤维羊全血.4.保存方法:经鉴定的 HP 菌种,于混合气体中(5%氧、85%氮、10%二氧化炭)37℃培养72小时,在布氏血平皿上可得到生长丰富的菌苔。然后,在含有0.4～0.5ml 新
Helicobacter Pylori (HP) is closely related to gastric diseases such as chronic gastritis and peptic ulcer, so it is of great significance to study the preservation of bacteria. To this end, we started from 89 to study the HP strain at -70 ℃, with fresh defibrillation sheep whole blood can survive for 14 months, and can maintain the typical bacterial morphology and the original biochemical reaction characteristics. MATERIALS AND METHODS 1. Strain: 10 strains of HP were obtained from gastroscopy biopsy of gastric mucosa specimens of various types of gastric patients, and were used as species for preservation after being passaged for generations. 2. Medium: The test used to improve Campy-Bap (Brinell agar), according to the relevant literature preparation. 3. Storage solution: fresh defibrinated sheep whole blood .4 preservation method: The identified HP strain cultured in a mixed gas (5% oxygen, 85% nitrogen, 10% carbon dioxide) at 37 ℃ for 72 hours, Brine blood plate can be obtained in the growth of rich lawn. Then, containing 0.4 ~ 0.5ml new