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为探讨背根节中神经生长因子（ＮＧＦ）及ｔｒｋＡ蛋白对脊髓可塑性的作用，采用成年大鼠５只，建立单侧备用根模型（即切断一侧Ｌ１～４，Ｌ６～Ｓ１的脊髓节段背根，保留Ｌ５为备用根及备用背根节）。术后动物存活５天，灌流固定，取双侧Ｌ５背根节（ＤＲＧ）冰冻切片，用兔抗２．５ＳＮＧＦ及ｔｒｋＡ蛋白多克隆抗体分别进行Ｓ－Ｐ法免疫组化反应，ＤＡＢ显色。并对免疫反应阳性神经元进行图像分析。其结果手术侧ＤＲＧ中ＮＧＦ及ｔｒｋＡ蛋白阳性神经元的平均灰度值均分别显著小于非手术侧（Ｐ＜０．０１），表明手术侧阳性神经元中ＮＧＦ及ｔｒｋＡ的含量增加。结果提示，备用背根节神经元中ＮＧＦ及ｔｒｋＡ蛋白表达的增多可能与部分去传入后脊髓的可塑性变化有关
In order to investigate the effect of nerve growth factor (NGF) and trkA protein on spinal cord plasticity in dorsal root ganglion, five adult rats were used to establish unilateral lateral root models (ie, spinal cord segments of L1 ~ 4, L6 ~ S1, Dorsal root, leaving L5 as spare root and spare dorsal root). The animals survived for 5 days and perfused fixedly. Frozen slices of bilateral DRG were collected. S-P immunohistochemical staining and DAB staining were performed with rabbit anti-2.5SNGF and trkA polyclonal antibody respectively. Immuno-positive neurons were imaged. The results showed that the average gray values of NGF and trkA positive neurons in DRG were significantly smaller than those in non-surgical side (P <0.01), which indicated that the content of NGF and trkA in the positive neurons increased. The results suggest that the increased expression of NGF and trkA in spare DRG neurons may be related to the plasticity of spinal cord after partial degeneration