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采用亲和层析法纯化的ＡＦＰ抗原免疫的ＢＡＬＢ／ｃ鼠脾细胞与ＳＰ２／０小鼠骨髓瘤细胞系融合，获三株分泌抗人ＡＦＰ单克隆抗体（ＭｃＡｂ）的杂交瘤细胞系．用ＥＬＩＳＡ阻断试验和夹心ＥＬＩＳＡ试验作特异性鉴定，杂交瘤细胞株染色体的众数为１０４条，三株细胞系ＭｃＡｂ均属小鼠ＩｇＧ１型．经体外连续传代３个月及冻存一年复苏后均能持续分泌抗ＡＦＰ抗体
The BALB / c mouse spleen cells immunized with the AFP antigen purified by affinity chromatography were fused with the SP2 / 0 mouse myeloma cell line and three hybridoma cell lines secreting anti-human AFP monoclonal antibody (McAb) were obtained. Specificity was identified by ELISA and sandwich ELISA. The number of chromosomes in the hybridoma cell line was 104, and all the three cell lines McAb were mouse IgG1. After continuous passage in vitro 3 months and cryopreservation after a year of recovery can continue to secrete anti-AFP antibody