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目的：了解肺癌细胞核ＤＮＡ倍性分布与肺癌临床病理类型（特征）的关系，研究测定癌旁细胞核ＤＮＡ倍性在判断肿瘤局部转移中的作用。方法：应用流式细胞仪（ＦＣＭ）测定手术切除的４８例新鲜肺癌组织标本，包括１６例癌旁肺组织细胞核ＤＮＡ倍体模式。结果：二倍体肿瘤主要分布于ＴＮＭ分期Ⅰ～Ⅱ期及分化好的Ⅰ～Ⅱ级肿瘤；随着肿瘤的病理分期或分级的发展，异倍体肿瘤的检出率逐渐上升，约７５％患者的肿瘤表现为异倍体ＤＮＡ类型。伴癌栓形成或淋巴结转移，Ⅲ～Ⅳ期肺癌其癌旁肺细胞核异倍体率有较明显升高。结论：测定癌及癌旁细胞核ＤＮＡ倍体模式，在评估肺癌恶性程度上有重要意义。
Objective: To understand the relationship between the ploidy distribution of lung cancer cells and the clinicopathological types (characteristics) of lung cancer, and to study the role of DNA ploidy in adjacent cancer cells in determining the local metastasis of tumor. Methods: Flow cytometry (FCM) was used to determine 48 cases of fresh lung cancer specimens, including DNA ploidy patterns in 16 cases of adjacent lung tissues. Results: The diploid tumors were mainly distributed in stage Ⅰ ~ Ⅱ of TNM staging and well differentiated stage Ⅰ ~ Ⅱ tumors. With the development of pathological staging or grading of tumors, the detection rate of aneuploid tumors gradually increased, about 75% The patient’s tumor presents as aneuploid DNA type. With the formation of thrombus or lymph node metastasis, Ⅲ ~ Ⅳ lung cancer adjacent lung cell nuclear aneuploidy rate was significantly higher. Conclusion: The determination of DNA ploidy patterns in cancer and para-cancerous cells is of great significance in assessing the malignant degree of lung cancer.