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目的 检测麦胚黄酮类提取物对人体乳腺髓样癌细胞株 BCap- 37凋亡的诱导作用 ,探讨其分子学机制。方法 用流式细胞仪检测麦胚黄酮类提取物对人体乳腺髓样癌细胞株BCap- 37细胞凋亡的诱导作用及对 bcl- 2基因蛋白表达的抑制作用。用光学显微镜观察凋亡细胞的形态学特征。结果 1 .麦胚黄酮类提取物主要阻断乳腺癌细胞由 G2 - M向 S期转变 ,并能诱导细胞发生凋亡 ,呈现剂量 -效应关系 ( P<0 .0 0 0 1 )。 2 .麦胚黄酮类提取物亦能明显抑制人体乳腺癌细胞株 BCap- 37中 bcl- 2基因的蛋白表达 ,其表达率与麦胚黄酮类提取物浓度成负相关 ( P<0 .0 1 )。3.显微镜下观察 ,可见乳腺癌细胞出现凋亡特征性形态学改变 :细胞体积缩小、胞浆浓缩、胞核固缩、染色质靠核膜凝集成不同形态 ,如马蹄型、月牙型、肾型 ,形成凋亡小体。结论 麦胚黄酮类提取物具有诱导人体乳腺髓样癌细胞株 BCap- 37凋亡的作用 ,bcl- 2蛋白表达水平的降低可能是促进细胞凋亡的重要条件
Objective To investigate the induction of apoptosis of human breast myeloid carcinoma cell line BCap-37 by flavonoid extracts of wheat germ and explore its molecular mechanism. Methods Flow cytometry was used to detect the apoptosis of human breast myeloid carcinoma cell line BCap-37 induced by flavonoid extracts of wheat germ and the inhibition of bcl-2 gene expression. The morphological features of apoptotic cells were observed with an optical microscope. Results 1. The extracts of flavonoids from wheat germs mainly blocked the transition from G2-M to S-phase of breast cancer cells and induced apoptosis of cells, showing a dose-effect relationship (P < 0.01). 2. The flavonoid extract of wheat germ can also significantly inhibit the protein expression of bcl-2 gene in human breast cancer cell line BCap-37, and the expression rate is negatively correlated with the concentration of flavonoid extract of wheat germ (P < 0.01). ). 3. Observed under the microscope, visible breast cancer cell apoptosis characteristic morphological changes: cell volume reduction, cytoplasm concentration, nucleus pyknosis, chromatin nuclear membrane aggregation into different forms, such as horseshoe, crescent, kidney Types form apoptotic bodies. Conclusion The extract of flavonoids from wheat germ can induce the apoptosis of human breast myeloid carcinoma cell line BCap-37. The decrease of bcl-2 protein expression may be an important condition for promoting apoptosis.