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【目的】建立一套大豆抗疫霉菌根腐病基因鉴别菌系,并用于分析大豆品种(系)是否含有抗病基因Rps1a、Rps1c和Rps1k。【方法】采用下胚轴接种法测定了125个大豆疫霉菌分离物的毒性组成,筛选了不同毒力公式的6个大豆疫霉菌并建立了该菌系,测定了黄淮地区55个主栽大豆品种(系)对该菌系的抗性并通过基因推导方法进行抗病基因分析。【结果】55个大豆品种(系)共产生18种反应型。抗病基因的推导结果表明,有2个品种可能含有Rps1c,没有鉴定到可能携带有Rps1a或Rps1k的大豆品种(系)。【结论】黄淮地区携带抗病基因Rps1a、Rps1c和Rps1k的大豆品种(系)并不多,且易感疫霉菌,因此需要及时进行抗性育种并积极推广。
【Objective】 The objective of this study was to establish a set of identification strains of soybean root rot disease resistance and to analyze whether soybean varieties Rps1a, Rps1c and Rps1k contain the disease resistance genes. 【Method】 Toxicity of 125 isolates of Phytophthora sojae was determined by hypocotyl inoculation method. Six Phytophthora sojae strains with different virulence formulas were screened out and the strains were identified. 55 main cultivars Resistance of soybean cultivars (lines) to this strain and analysis of disease resistance genes by gene inference method. 【Result】 Fifteen soybean cultivars (lines) produced 18 reaction types. The deduced results of the disease resistance genes showed that 2 of the cultivars might contain Rps1c and no soybean cultivars (lines) that could carry Rps1a or Rps1k were identified. 【Conclusion】 There are not many soybean cultivars (lines) carrying Rps1a, Rps1c and Rps1k in Huang-Huai region, and susceptible to Phytophthora infestans. Therefore, it is necessary to carry out resistance breeding in time and actively promote it.