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Elemental calcium plays an important role in human physiology.In order to study the relationship between Ca-intake,Ca-chemical formulation,and Ca-absorptivity,a balance experiment using a 41Ca tracer technique in SD rats was conducted to measure the endogenous fecal calcium and true absorption of calcium.Apparent absorption of calcium was measured as a control to the endogenous calcium labeling experiment.These results show that by using 41Ca labeled endogenous calcium in vivo,researchers could obtain the true calcium absorption data without extrinsic labeling.Therefore,the method was not affected by the chemical structure or type of calcium supplement and might be used in evaluating the absorptivity of marketed calcium supplements.