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组蛋白H3与H2A、H2B、H4共同形成真核生物染色质核小体的八聚体核心。本文通过RACE的方法从休眠期的油桃花芽中克隆到一个编码组蛋白H3变体的基因,命名为PpH3.3。序列分析结果表明,该基因全长821bp,存在一个完整的开放阅读框411bp,编码136个氨基酸。预测的氨基酸序列具有H3家族典型的保守结构域,与拟南芥、葡萄的组蛋白H3,具有较高的相似性。利用荧光定量PCR技术分析该基因在花芽休眠进程中的表达特异性,发现PpH3.3在休眠期表达水平显著高于休眠诱导期和休眠解除期,并且单氰胺和高温处理能够诱导类似的表达模式。
Histone H3 together with H2A, H2B and H4 form the octamer core of eukaryotic chromatin nucleosomes. In this paper, we cloned a gene encoding Histone H3 variant from dormant nectarine flower bud by RACE and named it PpH3.3. Sequence analysis showed that the full length of this gene was 821bp. There was a complete 411bp open reading frame encoding 136 amino acids. The predicted amino acid sequence has a typical conserved domain of H3 family and has high similarity with histone H3 of Arabidopsis and grapevine. Fluorescence quantitative PCR was used to analyze the expression specificity of this gene during flower bud dormancy. The expression level of PpH3.3 during dormancy was significantly higher than that during dormancy induction and dormancy release, and similar effects could be induced by cyanamide and high temperature treatment mode.