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目的建立高效液相色谱-质谱联用(LC-MS-MS)法测定人血浆内多尼培南浓度的方法。方法采用Agilent Zorbax C18(2.1mm×150mm,5μm)色谱柱;柱温25℃;流动相为甲醇-水(含10mmol·L-1乙酸铵)(32∶68,V/V);流速为0.2mL·min-1;通过LC-MS-MS,电喷雾离子源(ESI),以多反应监测(MRM)方式进行检测;离子极性监测负离子(-);检测离子为多尼培南m/z419.8[M-H]-→331.4[M-H-C4H7O2]-,法罗培南(内标)m/z284.2[M-H]-→196.1[M-H-C4H7O2]-。结果多尼培南的最低定量限为3.003μg·L-1,线性范围为3.003～10010μg·L-1(r=0.9982)。结论该方法简便、灵敏度高,可以用来进行多尼培南的人体药动学和生物等效性研究。
OBJECTIVE To establish a method for the determination of Donepenem in human plasma by high performance liquid chromatography-mass spectrometry (LC-MS-MS). Methods The mobile phase was methanol-water (containing 10mmol·L-1 ammonium acetate) (32:68, V / V) at a flow rate of 0.2 mL · min-1; detected by multi-reaction monitoring (MRM) by LC-MS-MS and electrospray ionization source (ESI); ion polarity monitoring negative ions (-); z419.8 [MH] - → 331.4 [MH-C4H7O2] -, faropenem (internal standard) m / z 282.2 [MH] - → 196.1 [MH-C4H7O2] -. Results The lowest limit of quantification of donepezil was 3.003μg · L-1 with a linear range of 3.003 ~ 10010μg · L-1 (r = 0.9982). Conclusion The method is simple and sensitive and can be used to study the pharmacokinetics and bioequivalence of.