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目的为研制防治病毒性心肌炎的基因疫苗打下基础。方法以带有CVB。P1、P2区核酸序列的pGEM质粒为模板，用PCR方法克隆CVB3VP1基因cDNA，插入真核表达载体pcDNA3中的HindⅢ和XbaⅠ位点之间，构建成重组质粒。经大量扩增纯化后，肌肉注射免疫BALB／c小鼠3次，用CVB3毒株攻击小鼠，取血及脾细胞检测其免疫效果。结果小鼠经3次免疫后，虽未测出明显免疫应答指标，但诱导了T、B细胞的免疫记忆反应，免疫组鼠在CVB3毒株攻击后，迅速诱导产生了强烈的二次免疫应答。结论CVB3VP1基因疫苗对CVB3毒株的攻击有一定程度的保护作用。
Objective To lay the foundation for the development of gene vaccines against viral myocarditis. Method to have CVB. P1, P2 nucleic acid sequence of the pGEM plasmid template, cloned by PCR CVB3VP1 gene cDNA, inserted into the eukaryotic expression vector pcDNA3 Hind Ⅲ and Xba Ⅰ site between the constructed recombinant plasmid. After extensive amplification and purification, BALB / c mice were intramuscularly immunized three times, and the mice were challenged with CVB3 strain. Blood and spleen cells were collected to test the immune effect. RESULTS: After three immunizations, although no significant immune response was detected, the immunological memory responses of T and B cells were induced. After being challenged by CVB3, the immunized mice rapidly induced a strong secondary immune response . Conclusion CVB3VP1 gene vaccine has a certain degree of protection against the challenge of CVB3 strain.