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目的:研究醇沉处理和中药配伍对藜蒿叶水提物抑制白色念珠菌生长作用的影响。方法:采用微量液基稀释法测定不同醇沉浓度纯化处理后的藜蒿叶水提物对白色念珠菌的抑制作用,包括对其菌体生长量、生物膜形成、芽管生成作用等方面的影响,并考察中药配伍对藜蒿叶水提物抗白色念珠菌活性的影响。结果:醇沉处理可显著降低藜蒿叶水提物对白色念珠菌的最小抑菌浓度(MIC),醇沉浓度90%处理使MIC80(下降80%的药物质量浓度)由200 g·L~(-1)降至8 g·L~(-1),同时增强了其对白色念珠菌芽管生成和生物膜形成作用的抑制效果;在选取的18种常见植物药材中,薰衣草、黄芩、莪术对藜蒿叶水提物表现出了显著的抑菌增效作用(部分抑制浓度指数<0.5),与黄芩联用时藜蒿叶水提物的MIC80最小,达0.60 g·L~(-1)。结论:醇沉处理和中药配伍可显著提高藜蒿叶水提物对白色念珠菌的抑菌效果,为藜蒿副产物资源的综合利用提供参考。
OBJECTIVE: To study the effects of alcohol-sedimentation and compatibility of traditional Chinese medicine (TCM) on the inhibition of Candida albicans growth by aqueous extract of Artemisia selengensis. Methods: The inhibitory effects of aqueous extract of Artemisia selengensis leaf extract on Candida albicans were determined by the micro-liquid-base dilution method, including the effects on cell growth, biofilm formation and germ tube formation , And investigated the compatibility of traditional Chinese medicine on Artemisia selenge leaves aqueous extract of Candida albicans activity. Results: Alcohol precipitation treatment could significantly reduce the minimum inhibitory concentration (MIC) of aqueous extract of Artemisia selengensis on Candida albicans. Treatment with 90% alcohol concentration reduced the MIC80 (drug concentration 80%) from 200 g · L ~ (-1) -1) to 8 g · L -1, and at the same time enhanced the inhibitory effect on Candida albicans germ tube formation and biofilm formation. Of the 18 common botanicals selected, lavender, Scutellaria baicalensis, The aqueous extract of Artemisia selengensis exhibited a significant antibacterial and synergistic effect (partial inhibitory concentration index <0.5). When combined with Scutellaria baicalensis, aqueous extract of Artemisia selengensis had the lowest MIC80 of 0.60 g · L -1. Conclusion: The combination of alcohol precipitation and traditional Chinese medicine can significantly improve the antibacterial effect of Aqueous extract of Artemisia selengensis on Candida albicans, and provide a reference for the comprehensive utilization of the by-product resources of Artemisia selengensis.