论文部分内容阅读
目的:检测Nme2(nucleoside diphosphate kinase 2)基因在小鼠早期妊娠和人工诱导蜕膜化的子宫内膜组织中的表达规律;探究Nme2基因与子宫基质细胞蜕膜化的相关性。方法:利用免疫组织化学、Western blot和实时荧光定量PCR(q RTPCR)分别检测小鼠妊娠第5天着床点(D5IS)、着床旁(D5IIS),D6IS、D6IIS、D8IS、D8IIS及人工诱导蜕膜化模型小鼠子宫内膜中Nme2的表达定位,m RNA和蛋白质水平的表达水平。结果:小鼠D5、D6、D8子宫内膜中着床点Nme2基因的m RNA和蛋白水平的表达明显高于着床旁;免疫组织化学结果显示Nme2表达定位表达于D5着床点的初级蜕膜区(primary decidua region,PDZ)、D6和D8着床点的次级蜕膜区(secondary decidua region,SDZ);人工诱导蜕膜化模型中,Nme2基因的m RNA和蛋白质水平在诱导组的表达明显高于对照组。结论:Nme2基因的表达上调与小鼠基质细胞的蜕膜化过程相关。
OBJECTIVE: To detect the expression of Nme2 (nucleoside diphosphate kinase 2) gene in early pregnancy and artificially induced decidualized endometrium in mice and to explore the correlation between Nme2 gene and uterine stromal cell decidualization. Methods: D5IS, D5IIS, D6IS, D6IIS, D8IS and D8IIS were detected by immunohistochemistry, Western blot and real-time qRTPCR respectively. Expression and localization of Nme2 in endometrium of decidualized mouse model, expression levels of m RNA and protein. Results: The mRNA and protein levels of Nme2 in implantation sites of D5, D6 and D8 mice were significantly higher than those at the implantation site. Immunohistochemistry showed that Nme2 expression was localized at the primary shedding site of D5 implantation site The primary decidua region (PDZ) and the secondary decidua region (SDZ) at the D6 and D8 implantation sites. In the artificially induced decidualization model, the mRNA and protein levels of the Nme2 gene in the induced group The expression was significantly higher than the control group. Conclusion: The up-regulation of Nme2 gene is associated with the process of stromalization of mouse stromal cells.