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为克服白血病的内源性耐药,以表阿霉素为抗肿瘤药物,氨氯地平为凋亡诱导剂,地喹氯铵为靶向分子,构建了一种新的表阿霉素抗耐药性脂质体。研究目的是建立一种高效液相方法可同时测定该脂质体中表阿霉素、氨氯地平和地喹氯铵等三种成分。室温条件采用ODS色谱柱进行分离,流动相为乙腈-0.02 M磷酸二氢钠-三乙胺(34:66:0.3,v/v/v,用磷酸调pH值至4.0),检测波长为240 nm,流速为1.0 mL/min。在1～50μg/mL浓度范围内,表阿霉素、氨氯地平与地喹氯铵的浓度与峰面积之间分别呈现良好的线性关系(r~2=0.9999)。表阿霉素,氨氯地平与地喹氯铵的平均回收率分别为95.86%～97.52%,97.17%～98.92%和98.04%～101.13%;三批载药脂质体中表阿霉素、氨氯地平和地喹氯铵含量范围分别为(564.2～606.1)μg/mL、(641.0～704.0)μg/mL、(816.0～898.0)μg/mL。脂质体中表阿霉素和氨氯地平的包封率分别为90%左右,地喹氯铵的修饰率大约70μg/μmol磷脂。该方法简便、重现性好,可用于同时检测该脂质体中的表阿霉素、氨氯地平和地喹氯铵。
In order to overcome the endogenous resistance of leukemia, epirubicin was used as antitumor drug, amlodipine was an apoptosis inducer and dequalacetone was used as a target molecule to construct a new epirubicin resistance Medicinal liposomes. The purpose of this study was to establish an HPLC method for the simultaneous determination of three components of epirubicin, amlodipine and dequalinium chloride in the liposome. The mobile phase was acetonitrile-0.02 M monobasic sodium phosphate-triethylamine (34:66:.33, v / v / v, adjusted to pH 4.0 with phosphoric acid) by ODS column at room temperature. The detection wavelength was 240 nm at a flow rate of 1.0 mL / min. In the range of 1 ~ 50μg / mL, there was a good linear relationship between the concentration of epirubicin, amlodipine and dequalinium chloride and peak area (r ~ 2 = 0.9999). The average recoveries of epirubicin, amlodipine and dequalinium chloride ranged from 95.86% to 97.52%, from 97.17% to 98.92% and from 98.04% to 101.13%, respectively. The average recoveries of epirubicin, Amlodipine and dequalinium chloride ranged from 564.2 to 606.1 μg / mL, 641.0 to 704.0 μg / mL, and 816.0 to 898.0 μg / mL, respectively. The entrapment efficiencies of epirubicin and amlodipine in liposomes were about 90%, respectively, and that of dequalinium chloride was about 70μg / μmol phospholipid. The method is simple, reproducible and can be used for simultaneous detection of epirubicin, amlodipine and dequalinium chloride in the liposome.