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目的：了解冬凌草甲素（Ｏｒｉｄ）及其它香茶菜属二萜类化合物香茶菜醛（Ａｍｅ）毛叶香茶菜醇（Ｉｓｏ）是否与冬凌草甲素同样具有抗氧化作用。方法：用分光光度法测定脂质过氧化物丙二醛（ＭＤＡ）含量及线粒体肿胀度。用荧光分光光度法测定线粒体膜流动性。结果：Ｏｒｉｄ，Ａｍｅ，Ｉｓｏ４０，８０，１６０μｍｏｌ／Ｌ抑制铁半胱氨酸（Ｆｅ２＋Ｃｙｓ）引起的肝线粒体ＭＤＡ形成，并呈剂量－依赖关系。Ｏｒｉｄ１６０μｍｏｌ／Ｌ抑制肝线粒体膜流动性下降。（Ｐ值分别为０２９７±０．０２２，Ｆｅ２＋Ｃｙｓ的Ｐ值为０４２３±０．０１４）；Ａｍｅ１６０μｍｏｌ／Ｌ还可抑制脂质过氧化引起的肝线粒体肿胀。结论：Ａｍｅ，Ｉｓｏ与Ｏｒｉｄ同样可能通过抑制脂质过氧化而产生抗氧化作用。
OBJECTIVE: To investigate whether Orid and other Chaenomeles diterpenoids, Ame, are the same antioxidants as oridonin. Methods: The contents of malondialdehyde (MDA) and mitochondrial swelling were measured by spectrophotometry. Mitochondrial membrane fluidity was determined by fluorescence spectrophotometry. RESULTS: Orid, Ame, Iso40, 80 and 160μmol / L inhibited the formation of hepatic mitochondrial MDA induced by iron-cysteine (Fe2 + -Cys) in a dose-dependent manner. Orid160μmol / L inhibited the decrease of mitochondrial membrane fluidity in the liver. (P = 0297 ± 0.022, P = 02323 ± 0.014, respectively). Ame160μmol / L also inhibited hepatic mitochondria swelling induced by lipid peroxidation. Conclusion: Ame, Iso and Orid may also have antioxidant effects by inhibiting lipid peroxidation.