论文部分内容阅读
二化螟幼虫表皮在 Grace 培养液中经20-羟蜕皮酮诱导24h 后,移入不含20-羟蜕皮酮的 Grace 培养液中继续培养48h,出现表皮开裂及皮层溶离。而几丁质抑制剂 ATABRON则能抑制这种过程的发生。与传统的生物测定方法相比,用这种表皮培养系统检测几丁质抑制剂的生物活性,具有快速,灵敏的特点,并能从组织学水平直接观察到几丁质抑制剂对表皮蜕皮过程的影响。
After being induced by 20-hydroxyecdysone in Grace’s medium for 24 hours, the epidermis of the stem borer was transferred to Grace’s medium containing 20-hydroxyecdysone for 48 hours, resulting in epidermal dehiscence and cortex elution. The chitin inhibitor ATABRON can inhibit the occurrence of this process. Compared with the traditional bioassay methods, this epidermal culture system to detect the biological activity of chitin inhibitors has the characteristics of rapid and sensitive, and can be directly observed from the histological level of chitin inhibitors epidermal molting process Impact.