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Obejective The domainⅢof dengue virus type 2 envelope was cloned and expressed in Escherichia coli and the inhibited effects of recombinant protein on virus was detected.Methods In this study,the domainⅢ(DⅢ)protein of the dengue virus type-2(DENV-2)envelope(E)antigen was expressed in Escherichia coli by fusion with a carrier protein.The protein was purified using enzymatic cleavage and affinity purification.Rabbit immunization and antibody detection was carried out.Inhibition of DENV-2 infection was observed by DENV-2 EDⅢprotein and its immunity rabbits serum.Results The recombinant expression DENV-2 EDⅢprotein plasmid was constructed successfully.After isopropyl thiogalactoside induction,a specific soluble 29 kD protein was obtained,and the expression product
Obejective The domain III of dengue virus type 2 envelope was cloned and expressed in Escherichia coli and the inhibited effects of recombinant protein on virus was detected. Methods In this study, the domain III (DIII) protein of the dengue virus type-2 (DENV-2) envelope (E) antigen was expressed in Escherichia coli by fusion with a carrier protein. The protein was purified using enzymatic cleavage and affinity purification. Rabbit immunization and antibody detection was carried out. Inhibition of DENV-2 infection was observed by DENV-2 ED III protein and its immunity rabbits serum. Results The recombinant expression DENV-2 EDⅢprotein plasmid was constructed successfully. After isopropyl thiogalactoside induction, a specific soluble 29 kD protein was obtained, and the expression product