为了对膀胱癌进行早期的检测和治疗,运用免疫毒素寻找并有效杀死癌细胞不失为一个好方法.但是,早期的免疫毒素是通过化学偶联而成的,有以下缺点:1.易释放从而伤害了正常细胞.2 免疫毒素的免疫原性较强.且渗透性较差,导致毒素的活性不够强.为了克服上述缺点,我们利用DNA重组技术,将异源的SeFv基因与削减的毒素基因融合并克隆,在大肠杆菌中翻译出单链融合蛋白.这就是所谓的第三代免疫毒素,又称基因工程免疫毒素. In order to early detection and treatment of bladder cancer, the use of immunotoxins to find and effectively kill cancer cells is a good way.But, the early immunotoxins are chemically coupled and have the following disadvantages: 1. Easy to release Injuring normal cells.2 Immunotoxins have strong immunogenicity and poor permeability, resulting in not strong toxin activity.In order to overcome the above shortcomings, we used DNA recombination technology to combine the heterologous SeFv gene with the reduced toxin gene Fusion and cloning, in E. coli translated single-chain fusion protein.This is the so-called third-generation immunotoxin, also known as genetic engineering immunotoxin.