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【目的】研究大豆异黄酮与脂肪、蛋白质含量基因定位及相关性,为大豆品质改良、分子育种及基因克隆等应用提供理论依据。【方法】利用SSR技术,对晋豆23号和灰布支杂交构建的F13代大豆重组自交系群体的474个家系进行了连锁图谱的构建。在此基础上,利用WinQTLCart2.0软件分析了影响大豆异黄酮含量、脂肪含量和蛋白质含量3个重要品质性状的QTL,通过复合区间作图分析,检测QTL;同时,对异黄酮与脂肪、蛋白质的含量相关性分析。【结果】检测到23个QTL,其中控制异黄酮含量QTL有6个,分别定位在J、N、D2和G染色体的连锁群上;控制脂肪含量的QTL有11个,分别定位在第A1、A2、B2、C2和D2染色体的连锁群上;控制蛋白质含量的QTL有6个,分别定位在B2、C2、G和H1染色体的连锁群上。相关性分析结果表明:异黄酮与蛋白质含量呈极显著负相关;蛋白质和脂肪含量呈极显著负相关;蛋白质和蛋白质脂肪总量呈极显著正相关。【结论】3个重要品质性状的部分基因定位结果与其相关性分析是一致的,其结果对大豆品质育种应用有重要利用价值。
【Objective】 The objective of this study is to study the gene localization and correlation of soybean isoflavone, fat and protein content and provide theoretical basis for the application of soybean quality improvement, molecular breeding and gene cloning. 【Method】 The SSR technique was used to construct a linkage map of 474 lines of F13 generation of recombinant inbred lines of soybean inbred line Jindou 23 and Bianbuzhi. On this basis, QTLs affecting three important quality traits of soybean isoflavone content, fat content and protein content were analyzed by WinQTLCart2.0 software, and the QTLs were detected by composite interval mapping analysis. At the same time, the QTLs for isoflavone and fat, protein Correlation analysis. 【Result】 Twenty-three QTLs were detected, of which 6 were QTLs controlling isoflavone content and located on linkage groups J, N, D2 and G, respectively. There were 11 QTLs controlling lipid content, A2, B2, C2 and D2. Six QTLs controlling protein content were mapped on linkage groups B2, C2, G and H1 respectively. Correlation analysis showed that isoflavone had a significantly negative correlation with protein content, a significant negative correlation between protein and fat content, and a significant positive correlation between total protein and fat content. 【Conclusion】 The results of partial gene mapping of three important quality traits are consistent with their correlation analysis, and the results have important value for the application of soybean quality breeding.