论文部分内容阅读
目的:分析自身免疫性溶血性贫血(AIHA)患者交叉配血不合的原因,为保证患者及时得到输血治疗,在检测患者血样中ABO以外抗体后,针对性筛选相应抗原阴性血液。方法:ABO血型、Rh(D)血型及Rh表型鉴定采用试管法,抗体鉴定试验、直接抗人球蛋白法、交叉配血试验采用试管法和微柱凝胶法,综合判定结果。结果:AIHA患者含有自身抗体合并有抗-Mur、抗-E抗体,输注同型血液后,患者血红蛋白水平得到提升,疗效显著。结论:准确鉴定出患者血清中同时存在的自身抗体及抗-Mur、抗-E抗体,有利于缩短配血时间,筛选相应抗原阴性血液,保证输血安全有效。
OBJECTIVE: To analyze the causes of crossmatch incompatibility in patients with autoimmune hemolytic anemia (AIHA). In order to ensure that patients receive transfusion therapy in time, anti-ABO antibodies in blood samples of patients were screened and the corresponding antigen-negative blood was screened. Methods: The phenotypes of ABO blood group, Rh (D) blood group and Rh phenotype were determined by test tube method, antibody identification test, direct anti-human globulin method and cross-matching blood test by tube method and microcolumn gel method. Results: AIHA patients with autoantibodies combined with anti-Mur and anti-E antibodies, hemoglobin levels were elevated after infusion of the same type of blood, the effect was significant. Conclusion: The simultaneous identification of autoantibodies and anti-Mur and anti-E antibodies in the sera of patients is helpful to shorten the time of matching blood and screen the negative blood of the corresponding antigen to ensure the safe and effective blood transfusion.