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受体具有与配体(Ligands)相结合的能力,但酶也具有这种能力,其它物质如蛋白质、类脂质、神经节甙脂(Gangliosides)等也能在较弱程度上与配体相结合,所以,要证实一种受体的存在,这种结合就需满足以下一些标准: 标准: 1、这种结合一定要有高度亲和力。大多数受体反应的解离常数(Kd)都在100微微克分子(100pM)至10毫微克分子(10nM)之间。某些酶与配体的结合也有高度亲和力,这可用某些特异的酶抑制剂来作鉴别。这些酶抑制剂能减弱酶的活
Receptors have the ability to bind to Ligands, but enzymes also have this ability. Other substances such as proteins, lipids, gangliosides and the like can also interact with ligands to a lesser degree Therefore, to confirm the existence of a receptor, this combination must meet the following criteria: Standard: 1, this combination must have a high affinity. Most receptor reactions have dissociation constants (Kd) between 100 picomolar (100 pM) and 10 nanomolar (10 nM). Some enzymes also have a high affinity for ligand binding, which can be identified with certain specific enzyme inhibitors. These enzyme inhibitors can weaken the enzyme activity