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目的分析广西4地带绦虫分离株COX1序列,了解亚洲绦虫在广西的分布。方法对鹿寨、融水、田东、三江等4县现症病人进行驱虫,收集绦虫成虫。绦虫成虫经反复洗涤后,各取孕节一节,研碎后提取基因组DNA;同时设计特异性引物,PCR扩增各分离株COX1片段,对扩增产物进行T A克隆测序;应用相关软件分析序列的同源性、遗传距离,同时构建系统发生树。同时,从GenBank检索亚洲绦虫、猪带绦虫和牛带绦虫COX1基因序列,并与4地分离株基因序列进行比较。结果广西4地5个带绦虫分离株的COX1碱基序列长度均为444 bp。鹿寨株与亚洲绦虫之间基因差异为5个位点(1.11%),同源性为98.87%,遗传距离为0.011,其在邻接法和简约法构建的系统发生树中位于同一分枝。融水A株与猪带绦虫同源性达100%,B株与牛带绦虫和亚洲绦虫的同源性分别达98.20%和96.17%;田东和三江分离株与猪带绦虫的同源性分别为99.55%和96.40%,遗传距离分别为0.005和0.037;鹿寨株与牛带绦虫的同源性达96.40%。结论广西鹿寨县存在亚洲绦虫流行,融水县存在牛带绦虫和猪带绦虫混合流行。
Objective To analyze the COX1 sequence of the tapeworm isolates from Guangxi 4 and to understand the distribution of tapeworms in Guangxi. Methods Deworming, meltwater, Tiandong, Sanjiang and other 4 counties patients were deworming, collecting tapeworm adults. The tapeworm adults were washed repeatedly and each section of the embryo was removed and the genomic DNA was extracted after grinding. At the same time, specific primers were designed to amplify the COX1 fragments of each isolate, and the cloned products were cloned by TA cloning. The relevant software was used to analyze the sequence Homology, genetic distance, and phylogenetic tree. At the same time, the COX1 gene sequences of Taenia saginata, Taenia solium and Taenia saginatae were retrieved from GenBank and compared with those of 4 isolates. Results The COX1 nucleotide sequences of five Taenia isolates from four sites in Guangxi were 444 bp in length. There were 5 loci (1.11%) between Luzhai and Taenia asiatica, the homology was 98.87%, and the genetic distance was 0.011. It was located in the same branch in the phylogenetic tree constructed by the adjoining and parsimony methods. The homology between strain A and strain Taenia solium was 100%. The homologies between strain B and Taenia solium and Taenia saginata were 98.20% and 96.17%, respectively. 99.55% and 96.40%, respectively. The genetic distances were 0.005 and 0.037, respectively. The homology of Luzhai and Taenia saginata reached 96.40%. Conclusion There is a prevalence of tapeworm in Luzhai County, Guangxi Province. There is a mixed prevalence of Taenia solium and Taenia solium in Rongshui County.