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目的:观察大鼠脊髓后角浅层内δ阿片受体是否存在于初级传入C纤维内,为脑啡肽在痛觉初级传入调制中的突触前抑制机制提供更为可靠的形态学依据。材料与方法:应用双标(免疫)组织化学电镜技术标记大鼠脊髓后角浅层(Ⅰ～Ⅲ层)凝集素Ⅰ-B4结合位点和δ阿片受体。结果:大量轴突及轴突终末呈δ阿片受体阳性免疫反应,特别是在双标切片上,观察到约40％的凝集素Ⅰ-B4结合位点阳性纤维及其终未内也含有δ阿片受体。结论:脊髓后角浅层内的δ阿片受体部分存在于传递伤害性信息的初级传入C纤维中,以介导阿片物质脑啡肽对初级传入C纤维的突触前抑制作用。
OBJECTIVE: To observe whether δ opioid receptor is present in the primary afferent C fibers in the superficial layers of the rat spinal cord and provide a more reliable morphological basis for presynaptic inhibition of enkephalin in primary pain modulation . MATERIALS AND METHODS: I-B4 binding sites and δ opioid receptors of the lamina (Ⅰ ~ Ⅲ) in the spinal dorsal horn of spinal cord were labeled with double-labeled (immunohistochemistry) electron microscopy. RESULTS: The majority of axons and terminal axons showed a δ opioid receptor-positive immunoreactivity. In particular, about 40% of lectin Ⅰ-B4 binding site-positive fibers and their final δ opioid receptor. CONCLUSIONS: Delta opioid receptors in the superficial layers of the spinal cord reside in primary afferent C fibers that transmit nociceptive information to mediate presynaptic inhibition of opioid enkephalin on primary afferent C fibers.