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目的 :研究天津地区上呼吸道恶性淋巴瘤的临床病理、免疫表型及与 EB病毒的关系。方法 :应用单克隆抗体 U CHL - 1、CD3、L2 6 、4KB5 、CD79a、L CA、EMA、溶菌酶等抗体及 CS1- 4(L MP- 1)进行免疫组化染色 ,以进行淋巴瘤确诊、淋巴瘤免疫表型研究和 EB病毒感染情况的分析 ;EB病毒寡核苷酸探针 (EBER1/ 2 )原位杂交检测 ,探讨瘤细胞 EB病毒感染情况及与 EB病毒潜在膜蛋白表达的相关性。结果 :12 5例上呼吸道原诊断为恶性淋巴瘤和高度怀疑恶性淋巴瘤的病例中 112例确诊为淋巴瘤 ,诊断符合率 89.6 %。其中 6 6例为 T细胞性淋巴瘤 (占5 8.93% ) ,46例为 B细胞性淋巴瘤 (占 41.0 7% )。男性明显多于女性 (85例 / 2 7例 )。平均年龄 48.2 7岁 (10岁～ 88岁 )。 EBV- EBER1/ 2原位杂交检测 T细胞性淋巴瘤 34例 ,17例阳性 ,占 5 0 % ,阳性细胞占肿瘤细胞的 2 0 %～80 % ,EBV潜在膜蛋白 L MP- 1检测 5 3例 ,11例阳性 ,占 2 0 .75 % ,阳性率低于 EBER探针。结论 :天津地区上呼吸道恶性淋巴瘤以 T细胞性淋巴瘤为多见 ,发病可能与 EB病毒感染有关。
Objective: To study the clinicopathological features, immunophenotype and the relationship with Epstein-Barr virus in upper respiratory tract malignant lymphoma in Tianjin area. Methods: Immunohistochemical staining with monoclonal antibodies such as UCHL - 1, CD3, L2 6, 4KB5, CD79a, L CA, EMA, lysozyme and other antibodies and CS1-4 (L MP- 1) , Lymphoid phenotype and Epstein-Barr virus (EBV) infection. EBR1 / 2 in situ hybridization was used to detect the Epstein-Barr virus (EBV) infection in tumor cells and its correlation with Epstein-Barr virus latent membrane protein Sex. Results: One hundred and twelve cases diagnosed as lymphoma in 125 cases of upper respiratory tract original malignant lymphoma and highly suspected malignant lymphoma were diagnosed as lymphoma with a coincidence rate of 89.6%. Among them, 66 cases were T cell lymphoma (5 8.93%) and 46 cases were B cell lymphoma (41.0 7%). Significantly more males than females (85/27). The average age of 48.2 7 years old (10 years old to 88 years old). EBV-EBER1 / 2 in situ hybridization detected 34 cases of T cell lymphoma, 17 cases were positive, accounting for 50%, positive cells accounted for 20% to 80% of tumor cells, EBV potential membrane protein L MP-1 detection 5 3 Cases, 11 cases were positive, accounting for 20.7%, the positive rate is lower than the EBER probe. Conclusion: T-lymphoma is a common malignant lymphoma of upper respiratory tract in Tianjin. The incidence may be related to Epstein-Barr virus infection.