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目的采用腹部结扎大鼠胸导管,建立胰淋巴瘀滞动物模型,观察实验动物胰组织结构变化和胰淀肽沉积情况,探讨实验性淋巴瘀滞对胰组织细微结构的影响。方法 20只10月龄SD大鼠随机分为实验(手术)组和对照(假性手术)组;造模后6个月取材,部分胰腺标本行石蜡包埋和组织切片,行常规HE和刚果红染色后光镜观察;部分标本做冰冻切片,进行免疫组织化学染色和光镜观察。结果①实验组大鼠胰组织切片HE染色光镜观察显示,胰腺小叶间隙显著增宽,出现明显的淋巴管扩张、结缔组织增生和脂肪堆积,可见胰岛呈明显淡染。②术后动物刚果红染色切片光镜观察提示,胰腺小叶组织间隙明显增宽,胰岛表现为朱红色刚果红着色现象。③胰淀肽免疫组织化学染色切片光镜观察表明,在术后动物的胰岛及其周围,呈现胰淀肽免疫反应强阳性棕褐色着色反应。结论实验动物胰组织结构的形态学变化提示,胸导管结扎可导致胰淋巴引流障碍,引起胰淋巴管扩张、结缔组织间隙增宽、脂肪堆积以及胰淀肽沉积等,这些结构变化可能影响胰岛的内分泌功能。
Objective To establish a model of pancreas-lymphatic stasis in rats by ligating the thoracic duct of the abdomen and observing the changes of pancreatic tissue structure and the deposition of pancreatic amylolide in experimental animals to investigate the effects of experimental lymphatic stasis on the microstructure of pancreatic tissue. Methods Twenty SD rats of 10 months old were randomly divided into experimental group and control group (sham operation). Six months after modeling, some of the pancreas specimens were paraffin-embedded and sectioned. Conventional HE and Congo Observed by light microscope after red staining; some specimens were frozen sections, immunohistochemical staining and light microscopy. Results ① The pancreatic tissue sections of the experimental group were observed by light microscopy with HE staining. The small interlobular space of the pancreas was significantly broadened, and lymphatic dilation, connective tissue hyperplasia and accumulation of fat were observed. ② postoperative animal Congo red stained sections observed by light microscopy showed that the gap was significantly enlarged pancreatic lobules, pancreatic islet scarlet Congo red staining. ③ pancreatic peptide immunohistochemical staining sections observed by light microscopy showed that in the postoperative animals and its islets around the pancreas peptide immunoreactivity strong positive tan staining reaction. Conclusion The morphological changes of pancreatic tissue in experimental animals suggest that the thoracic duct ligation can lead to pancreatic and lymphatic drainage disorders, causing the expansion of pancreatic and lymphatic vessels, the widening of the connective tissue gap, the accumulation of fat and the deposition of pancreatic peptides. These structural changes may affect the islet Endocrine function.