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作者采用福尔马林固定,石蜡包埋切片,用抗角蛋白抗体对原发限局性皮肤淀粉样变(AD)及其他类型淀粉样变中的淀粉样蛋白进行免疫组化研究。选择9例原发限局性皮肤淀粉样变,8例系统性淀粉样变和5名正常人皮肤标本以及3种抗体:单克隆抗角蛋白抗体EAB—903和EAB—904,以及二种单克隆抗角蛋白抗体的混合抗体MAK—6,用ABC方法进行染色。并用连续切片Dylon染色以证实在这些切片上有淀粉样蛋白的存在。
The authors used formalin-fixed, paraffin-embedded sections and immunohistochemistry of amyloid in primary cutaneous amyloidosis (AD) and other types of amyloidosis with anti-keratin antibodies. Nine primary cutaneous amyloidosis, eight systemic amyloidosis and five normal human skin specimens as well as three antibodies: monoclonal anti-keratin antibodies EAB-903 and EAB-904, as well as two monoclonal The anti-keratin antibody mixed antibody MAK-6 was stained by the ABC method. And stained with serial sections Dylon to confirm the presence of amyloid on these sections.