为探讨不同标记方式对制备免疫PCR指示分子的影响,首先制备了一个用于免疫PCR指示分子DNA片段的重组质粒（腺病毒六邻体基因片段）,用生物素-dUTP分别以不同的方式标记DNA:PCR产物标记、PCR直接标记、线性重级质粒标记和回收重组质粒插入片段的标记。结果表明,以线性重组质粒标记生物素制备的指示分子具有最佳的效果。 In order to explore the effect of different labeling methods on the preparation of immunological PCR indicator molecules, a recombinant plasmid (adenovirus hexon gene fragment) for immunizing PCR indicator DNA fragments was prepared and labeled with biotin-dUTP in different ways DNA: PCR product marker, PCR direct marker, linear heavy-grade plasmid marker, and label for recovery of recombinant plasmid insert. The results showed that the linear recombinant plasmid labeled biotin prepared indicator molecules with the best results.