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目的探讨抗辐灵对微波辐射后大鼠免疫损伤的影响。方法采用30 mW/cm~2的微波辐射120只二级雄性Wistar大鼠,各组大鼠于辐射后当天开始,每天1次,连续14 d。药物组给予抗辐灵,药物浓度分别为0.75 g/kg/d、1.5 g/kg/d、3 g/kg/d,安多霖药物组给予安多霖,药物浓度为1.5 g/kg/d,正常对照组和辐射对照组均给予等比体积的蒸馏水。各组大鼠分别于辐射后7 d(给药7 d)、14 d(停药后6 h)和21 d(停药后7 d)抽取外周血,应用全自动血细胞分析仪检测白细胞(white blood cell,WBC)计数、淋巴细胞计数及比例(lymphocyte count,LYM)、红细胞(red blood cell,RBC)计数、血红蛋白(hemoglobin,Hb)和血小板总数(platelet,PLT)等血液细胞学指标,应用流式细胞仪检测CD_4~+/CD_8~+比值。结果 30 mW/cm~2辐射后14 d(停药后6 h),辐射对照组与正常对照组比,大鼠外周血WBC有降低趋势;与辐射对照组比,抗辐灵药物组大鼠外周血WBC明显升高(P<0.05);辐射后7 d,辐射对照组和安多霖药物组大鼠外周血RBC较正常对照组明显升高(P<0.05),抗辐灵药物组与正常对照组相比无显著性差异,与辐射对照组和安多霖药物组相比显著减少(P<0.05或0.01);辐射后14 d(停药后6 h),与正常对照组相比,辐射对照组和安多霖药物组LYM明显降低(P<0.05);与辐射对照组相比,抗辐灵药物组LYM明显升高(P<0.05);与安多霖药物组相比,1.5 g/kg/d和3 g/kg/d抗辐灵药物组LYM明显升高(P<0.05);辐射后各时间点,各组之间HGB和PLT无显著性差异。辐射后7 d(给药7 d),辐射对照组CD_4~+/CD_8~+比值明显降低(P<0.05)。结论抗辐灵对30 mW/cm~2微波辐射引起的大鼠免疫损伤具有保护作用,效果优于安多霖,1.5 g/kg/d为最佳剂量。
Objective To investigate the effect of Kang Ling on the immunological injury of rats after microwave radiation. Methods A total of 120 second-grade male Wistar rats were irradiated with 30 mW / cm ~ (-2) microwaves. The rats in each group started on the day after the irradiation, once a day for 14 days. Drug group given anti-radiation Ling, drug concentrations were 0.75 g / kg / d, 1.5 g / kg / d, 3 g / kg / d, Andolimin group was given Andorraine, drug concentration 1.5 g / kg / d, normal control group and radiation control group were given equal volume of distilled water. Peripheral blood was drawn on 7 d (7 d after dosing), 14 d after dosing (6 h after dosing) and 21 d after dosing (7 d after dosing), and the white blood cells blood cell (WBC) count, lymphocyte count (LYM), red blood cell count (RBC), hemoglobin (Hb) and platelet (PLT) Flow cytometry CD_4 ~ + / CD_8 ~ + ratio. Results Compared with the normal control group, the WBC in the peripheral blood of the irradiated control group was lower than that of the normal control group at 14 d after radiation (6 h after stopping the treatment). Compared with the radiation control group, (P <0.05). At 7 d after irradiation, RBC in peripheral blood of radiation control group and Andoline drug group was significantly higher than that of normal control group (P <0.05) There was no significant difference between the normal control group and the control group (P <0.05 or 0.01), while the control group was significantly reduced (P <0.05 or 0.01) (P <0.05). Compared with radiation control group, the LYM of Radix-treated group was significantly increased (P <0.05). Compared with that of Andolimycin-treated group, The LYM in 1.5 g / kg / d and 3 g / kg / d anti-radiation drug groups were significantly increased (P <0.05). There were no significant differences in HGB and PLT between groups at all time points after irradiation. The ratio of CD_4 ~ + / CD_8 ~ + in the radiation control group decreased significantly (P <0.05) on day 7 after irradiation (for 7 days). Conclusion Radix Linguisticin has a protective effect on immune damage induced by 30 mW / cm ~ 2 microwave irradiation in rats, and its effect is better than that of Andorol, 1.5 g / kg / d is the best dose.