获得纯菌种后,一般都要转接试管扩制母种。在农村买试管较难,而酒瓶到处都有。为此,我从1983年起用酒瓶代试管制种,取得了较好效果。具体做法:取土豆(去皮切细)200克,葡萄糖20克,琼脂20克,磷酸二氢钾3克,硫酸镁1.6克,维生素B_1 10毫克,水1000毫升,配制成PDA培养基,分装于20只酒瓶中,每瓶60毫升。以15磅压力灭菌 Pure strains obtained, the general should be transferred to test tube expansion mother. It is harder to buy a test tube in the countryside, and bottles are everywhere. To this end, I started from 1983 with the bottle test tube species, and achieved good results. Specific practices: Take potato (peeled and shredded) 200 grams, 20 grams of glucose, 20 grams of agar, potassium dihydrogen phosphate 3 grams, 1.6 grams of magnesium sulfate, vitamin B_1 10 mg, 1000 ml of water, formulated as PDA medium, points Packed in 20 bottles, 60 ml each. Sterilize at 15 pounds