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Lusi Ces A1是亚麻纤维素生物合成途径中的关键基因。在已报道部分序列的基础上,采用特异引物的设计进行高保真PCR扩增并测序,获得亚麻Lusi Ces A1基因的全长序列,该基因序列长3 225bp,并通过交错延伸PCR技术进行了验证。该研究为亚麻Lusi Ces A1基因的结构和功能分析奠定了基础。
Lusi Ces A1 is a key gene in the cellulose biosynthesis pathway. Based on the reported partial sequences, the full-length sequence of Lusi Ces A1 gene of flax was obtained by high-fidelity PCR amplification and sequencing using specific primer design. The gene sequence was 3 225 bp in length and verified by cross-extension PCR . This study lays the foundation for the structural and functional analysis of the Lusi Ces A1 gene in flax.