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目的:探讨B细胞易位基因1(BTG1)在喉鳞状细胞癌组织中的表达及其与喉鳞状细胞癌临床病理特征及生物学行为的关系。方法:采用免疫组织化学和Western blot检测70例喉鳞状细胞癌组织和35例癌旁组织中BTG1蛋白的表达水平。结果:BTG1蛋白在喉癌组织中的阳性表达率为31.43%,明显低于癌旁组织(91.43%,P<0.05)。Western blot示喉癌和癌旁组织BTG1相对表达量分别为0.217±0.032和0.918±0.081(P<0.05)。BTG1在喉鳞状细胞癌组织中蛋白的表达与患者性别、年龄、肿瘤所在部位无关,与肿瘤局部浸润深度、区域淋巴结转移、临床分期、组织学分级呈负相关(P<0.05或P<0.01)。结论:在喉鳞状细胞癌组织中,BTG1蛋白的表达水平明显降低,检测BTG1的表达对喉癌的临床治疗和预后判断有一定的参考价值。
Objective: To investigate the expression of B cell translocation gene 1 (BTG1) in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinicopathological features and biological behavior of laryngeal squamous cell carcinoma (LSCC). Methods: The expressions of BTG1 protein in 70 cases of laryngeal squamous cell carcinoma and 35 cases of adjacent tissues were detected by immunohistochemistry and Western blot. Results: The positive expression rate of BTG1 in laryngeal carcinoma was 31.43%, which was significantly lower than that in paracancerous tissues (91.43%, P <0.05). Western blot showed that the relative expression levels of BTG1 in laryngeal carcinoma and adjacent normal tissues were 0.217 ± 0.032 and 0.918 ± 0.081 (P <0.05), respectively. The expression of BTG1 in laryngeal squamous cell carcinoma was negatively correlated with the gender, age and tumor location (P <0.05 or P <0.01), but not with the depth of tumor invasion, regional lymph node metastasis, clinical stage and histological grade ). CONCLUSION: The expression of BTG1 protein in laryngeal squamous cell carcinoma is significantly decreased, and the expression of BTG1 can be used as reference for the clinical treatment and prognosis of laryngeal carcinoma.