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目的:研究姜黄素对SiO2所致小鼠矽肺模型血清及肺组织中肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)表达的影响。方法:将实验小鼠随机分为5组,即假手术组、模型组、姜黄素干预组(高、中、低剂量)。于姜黄素干预第14天、42天后分别每组各处死6、9只,采用ELISA法测定肺组织和血清中TNF-α、TGF-β1的含量。结果:模型组的肺组织炎症反应、纤维化程度较明显;与假手术组比较,肺组织匀浆、血清中TGF-β1、TNF-α含量均明显升高(P<0.01)。给予姜黄素干预后,可以不同程度地降低肺组织匀浆及血清中TGF-β1、TNF-α的含量(P<0.05),同时纤维化程度减轻。结论:姜黄素能降低SiO2致小鼠矽肺模型肺组织和血清中TNF-α、TGF-β1水平,可能通过下调上述细胞因子水平而发挥抗肺纤维化作用。
Objective: To investigate the effects of curcumin on the expression of tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in serum and lung tissue of silicosis model mice induced by SiO2. Methods: The experimental mice were randomly divided into 5 groups: sham operation group, model group, and curcumin intervention group (high, middle and low doses). After the intervention of curcumin for 14 days and 42 days, 6 and 9 mice were killed in each group. The levels of TNF-α and TGF-β1 in lung tissue and serum were determined by ELISA. Results: The inflammatory reaction and fibrosis of the lung tissue in the model group were obvious. Compared with the sham group, the levels of TGF-β1 and TNF-α in the lung homogenate and serum were significantly increased (P<0.01). After intervention with curcumin, the contents of TGF-β1 and TNF-α in serum and serum homogenate could be decreased to varying degrees (P<0.05), and the degree of fibrosis was reduced. Conclusion: Curcumin can reduce the levels of TNF-α and TGF-β1 in lung tissue and serum of SiO2-induced silicosis models, and may play a role in anti-lung fibrosis by down-regulating the levels of these cytokines.