论文部分内容阅读
本实验观察了Ａ－ＬＡＫ细胞的体外扩增及其抗肿瘤作用，并与ＬＡＫ细胞进行了比较。结果表明，Ａ－ＬＡＫ细胞在短期内大量扩增，在培养第７天时达到增殖高峰，Ａ－ＬＡＫ细胞增加１２．０２倍，而ＬＡＫ细胞增加２．６９倍，培养３周后Ａ－ＬＡＫ细胞增加５３．５０倍，而ＬＡＫ细胞仅增加４．４５倍。１８小时ＬＤＨ－Ｌ释放试验表明，Ａ－ＬＡＫ细胞对Ａｎｉｐ９７３和Ｋ５６２细胞均显示较高的杀伤活性，与ＬＡＫ细胞相比有显著差异（Ｐ＜０．０５）。通过ＦＡＣＳ细胞表型分析显示，Ａ－ＬＡＫ细胞大量表达ＣＤ１６、ＣＤ５６抗原，提示Ａ－ＬＡＫ细胞可能来源于ＬＧＬ－ＮＫ细胞群。
In this experiment, the in vitro expansion of A-LAK cells and its anti-tumor effect were observed and compared with LAK cells. The results showed that A-LAK cells expanded rapidly in the short-term, reached the peak of proliferation on the 7th day of culture, A-LAK cells increased 12.02-fold, while LAK cells increased 2.69-fold, and A-LAK was cultured after 3 weeks. The cells increased 53.50-fold while LAK cells only increased 4.45-fold. The 18-hour LDH-L release assay showed that A-LAK cells showed higher killing activity against Anip973 and K562 cells, which was significantly different from that of LAK cells (P<0.05). The phenotypic analysis of FACS cells showed that A-LAK cells express a large number of CD16 and CD56 antigens, suggesting that A-LAK cells may be derived from LGL-NK cell population.