论文部分内容阅读
目的研究星形胶质细胞中缝隙连接蛋白connexin 43(Cx43)的表达及由其形成的缝隙连接通讯功能的药物调控。方法实验分为正常对照组、全反式维甲酸组(10μmol/L全反式维甲酸作用24 h)及油酸酰胺组(25μmol/L油酸酰胺作用2 h)。western blotting法检测各组星形胶质细胞中Cx43总蛋白的表达;细胞免疫荧光法检测各组星形胶质细胞Cx43胞膜蛋白的表达;荧光示踪法检测各组星形胶质细胞的缝隙连接通讯功能。结果与正常对照组相比,全反式维甲酸能增强星形胶质细胞中Cx43总蛋白的表达(P<0.01),油酸酰胺则能减弱其表达(P<0.01);全反式维甲酸能增强Cx43胞膜蛋白表达,油酸酰胺能减弱其表达;全反式维甲酸能增强星形胶质细胞缝隙连接通讯功能(P<0.01),而油酸酰胺则可显著降低该功能(P<0.01)。结论药物全反式维甲酸及油酸酰胺可以对星形胶质细胞缝隙连接通讯功能进行调控,其机制可能与其影响星形胶质细胞Cx43总蛋白和胞膜蛋白的表达有关。
Objective To study the expression of connexin 43 (Cx43) in astrocytes and the drug regulation of the gap junctional communication function. Methods The experiment was divided into normal control group, all-trans retinoic acid group (24 h of 10 μmol / L all-trans retinoic acid) and oleic acid amide group (25 μmol / L of oleic acid amide for 2 h). Western blotting was used to detect the expression of total Cx43 protein in astrocytes; the expression of Cx43 protein in astrocytes was detected by immunofluorescence staining; the expression of astrocytes Gap connection communication function. Results Compared with normal control group, all-trans retinoic acid increased the expression of Cx43 protein in astrocytes (P <0.01), and decreased the expression of oleic acid amide (P <0.01). All-trans retinoic acid Formic acid increased Cx43 cell membrane protein expression, and oleic acid amide attenuated its expression. All-trans retinoic acid enhanced the gap junctional communication (P <0.01), while oleic acid amide reduced this function P <0.01). Conclusion All-trans retinoic acid and oleic acid amide can regulate the gap junctional communication of astrocytes. The mechanism may be related to the effects on the expression of Cx43 protein and membrane protein in astrocytes.