,The DOF Protein DAG1 and the DELLA Protein GAI Cooperate in Negatively Regulating the AtGA3ox1 Gene

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Dear Editor,Seed germination is controlled by multiple endogenous and environmental factors,which are integrated to trigger this developmental process at the right time.Gibberellins (GAs) are known to induce this process,and the levels of GAs are modulated by light-one of the most important environmental factors affecting seed germination.The bHLH transcription factor PIL5 (PHYTOCHROME INTERACTING FACTOR 3-LIKE 5) is the master repressor of light-mediated seed germination in Arabidopsis (Oh et al.,2004).In seeds kept in the dark,PIL5 activates transcription of the GAI (GA INSENSITIVE) gene (Peng et al.,1997;Oh et al.,2007),a DELLA transcriptional regulator that represses GA-mediated processes (Sun,2011).GAI plays a role in many growth processes with both unique and overlapping functions with another DELLA protein:RGA (REPRESSOR OF ga1-3) (Dill and Sun,2001).
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