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采用辐照法制备交联聚丙烯酰胺毛细管电泳涂层柱。毛细管先经双功能团试剂γ－ 甲基丙烯基氧丙基三甲氧基硅烷预处理，然后柱内充入丙烯酰胺溶液进行辐照聚合，得到线 性涂层柱；再经动态涂覆交联剂甲叉双丙烯酰胺，二次辐照交联，采用随机试验设计方法，对 辐照剂量率、辐照时间和交联剂浓度几个因素进行了优化。在ｐＨ ４、３３３ Ｖ／ｃｍ场强下，碱性 和酸性蛋白的平均柱效可达９．５ ｘ１０５和１．６９ｘ１０５理论塔板／米。对不同场强下的柱效、迁移 时间和分高度进行了研究。
Preparation of crosslinked polyacrylamide capillary electrophoresis coated column by irradiation. The capillary was first pretreated with γ-methacryloxypropyltrimethoxysilane by a bifunctional reagent, then the column was filled with an acrylamide solution to conduct irradiation polymerization to obtain a linear coating column; and then a dynamic coating of a crosslinking agent Methylene bisacrylamide, secondary radiation cross-linking, a randomized trial design method, the radiation dose rate, irradiation time and cross-linking agent concentration of several factors were optimized. At pH 4,333 V / cm field strength, the average efficiencies of basic and acidic proteins can reach 9.5 x 105 and 1.69 × 105 theoretical plates per meter. The effects of different field strength, migration time and sub-height were studied.