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目的 :评价高效液相色谱柱切换法在测定体液中 5 -氟尿嘧啶 (5FU)浓度的优点。方法 :高效液相色谱柱切换法测定血及腹腔液中 5FU浓度。结果 :标准曲线的回归方程为Y =9 6 9× 10 -6X - 1 33 ,r =0 9998,P <0 0 0 0 1;体液测定 5FU浓度的RSD为 0 2 %～ 5 6 % ,但是浓度小于或等于 0 0 5 μg·mL-1时 ,RSD为15 7%。体液中 5FU的回收率为 88 87%～ 116 8%。测定的最小浓度为 0 0 5 μg·mL-1。结论 :高效液相色谱柱切换法有样品处理简捷快速 ,灵敏度高 ,重复性好等优点 ;在临床药物监测时 ,可以起主要的作用
OBJECTIVE: To evaluate the advantages of high performance liquid chromatography (HPLC) column switching assay in the determination of 5 - fluorouracil (5FU) in body fluid. Methods: The concentration of 5FU in blood and peritoneal fluid was determined by high performance liquid chromatography (HPLC) column switch method. Results: The regression equation of the standard curve was Y = 9 6 9 × 10 -6 X - 1 33, r = 0 9998, P <0 0 0 0 1. The RSD of 5FU concentration in body fluid was 0.2% ~ 56% When the concentration was less than or equal to 0 0 5 μg · mL-1, the RSD was 15 7%. The recovery of 5FU in body fluids was 88 87% to 116 8%. The minimum concentration was 0 0 5 μg · mL-1. Conclusion: HPLC method has the advantages of simple and rapid sample processing, high sensitivity and good repeatability. It can play a major role in clinical drug monitoring