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本研究以芥菜型油菜(Brassica juncea L.)核不育系1161A和温敏雄性不育系K121S的BCF1为作图群体，采用混合群体分组分析法(bulked segregant analysis, BSA)定位油菜温敏雄性不育系K121S的育性转换基因(Fc)。研究结果表明，从86对多态性SSR引物中获得了5个连锁标记：Ol11-G11a、Na10-F06、Na14-G10、CN48和BrGMS961，其中Ol11-G11a、Na14-G10和Na10-F06等3个SSR标记与K121S育性转换基因的遗传距离较近，分别为1.6 cM、11.0 cM和19.7 cM。本研究结果可为油菜温敏雄性不育系K121S的育性转换基因的图位克隆及其利用提供依据。“,”In this study, the BC F1 derived from the cross between rapeseed nucleic male sterile line 1161A (Brassica juncea L.) and rapeseed hermo-sensitive genic male sterile line K121S (Brassica juncea L.) was used as mapping population, and the locus of the fertility conversion gene (Fc) in K121S was mapped by bulked-segregant analysis (BSA). The result indicated that five linked markers, including Ol11-G11a, Na10-F06, Na14-G10, CN48 and BrGMS961, were screened from the 86 polymorphic SSR markers, which of the genetic distance of Ol11-G11a, Na14-G10 and Na10-F06 are close to the Fc with 1.6 cM, 11.0 cM, 19.7 cM, respectively. It would be helpful for Fc positional cloning, and its utilizing.