目的探讨急性脑梗死患者溶栓治疗前后血浆 (NO)、肿瘤坏死因子 (TNF-α)、可溶性白细胞介素 2受体 (s IL-2 R)含量的变化。方法采用硝酸还原酶法检测 NO,双抗体夹心 EL ISA法检测 TNF-α和 s IL-2 R。结果 35例患者溶栓治疗前与对照组比较 :血浆 NO含量降低 (P<0 .0 5 ) ,TNF-α明显增高 ,s IL-2 R稍有升高 ,但无统计学差异。溶栓 2 4小时后与治疗前自身比较 :NO升高 P<0 .0 5 ,趋于恢复正常 ,TNF-α,s IL -2 R仍保持高水平状态 (P>0 .0 5 )。结论 NO、TNF-α、s IL -2 R参与了急性脑梗死病理生理过程 ,及时监测 NO含量变化 ,抑制 TNF-α、s IL -2 R的影响 ,对减轻脑梗死继发性脑损害有重要意义 Objective To investigate the changes of plasma (NO), tumor necrosis factor (TNF-α) and soluble interleukin-2 receptor (sIL-2R) in patients with acute cerebral infarction before thrombolysis. Methods Nitric acid reductase method was used to detect NO. TNF-α and sIL-2R were detected by double antibody sandwich ELISA. Results Compared with the control group, 35 patients before thrombolysis: the content of NO in plasma decreased (P <0.05), the level of TNF-α increased obviously, the level of IL-2R increased slightly, but there was no statistical difference. Thrombolytic 2 4 hours after treatment compared with their own: NO increased P <0. 05, tends to return to normal, TNF-α, s IL -2 R remains high (P> 0.05). Conclusion NO, TNF-αand IL-2R are involved in the pathophysiological process of acute cerebral infarction. The changes of NO content, the inhibition of TNF-αand IL-2R in time and the reduction of secondary brain damage Significance