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目的:探讨林可霉素(lin)对树突状细胞(DCs)系DC2.4免疫功能的影响。方法:设立3个组:DC2.4细胞组,DC2.4细胞+LPS组及DC2.4细胞+LPS+lin组(LPS及lin均为500 ng/mL)。在倒置显微镜下观察各实验组中DC2.4细胞的形态学变化。同时采用流式细胞仪分析DC2.4细胞表面标志MHC-Ⅱ类分子、CD86和CD80的表达。采用同种异体混合淋巴细胞反应(MLR)检测各实验组中DC2.4细胞刺激同种异体T淋巴细胞增殖的能力。用ELISA试剂盒检测干扰素-γ(IFN-γ)水平的变化。结果:倒置显微镜下显示,DC2.4细胞组为未成熟DCs形态,DC2.4细胞+LPS组及DC2.4细胞+LPS+lin两组均为成熟DCs的形态。流式细胞术分析证实,500 ng/mL LPS可以促进DC2.4细胞表面MHC-Ⅱ类分子、CD86和CD80的表达,并增强其刺激T细胞增殖及IFN-γ的分泌。但500 ng/mL lin联合500 ng/mL LPS能够部分抑制DC2.4细胞免疫调节作用。结论:Lin可以部分抑制成熟DC2.4细胞的免疫调节功能。
Objective: To investigate the effect of lincomycin on immune function of dendritic cells (DCs) DC2.4. Methods: Three groups were established: DC2.4 cells group, DC2.4 cells + LPS group and DC2.4 cells + LPS + lin group (500 ng / mL for both LPS and lin). The morphological changes of DC2.4 cells in each experimental group were observed under an inverted microscope. Meanwhile, the expression of MHC class Ⅱ molecules, CD86 and CD80 on the surface of DC2.4 cells was analyzed by flow cytometry. Allogeneic mixed lymphocyte reaction (MLR) was used to detect the ability of DC2.4 cells to stimulate allogeneic T lymphocyte proliferation in each experimental group. Changes in the level of interferon-γ (IFN-γ) were measured using an ELISA kit. Results: Under inverted microscope, DC2.4 cells were immature DCs morphology, DC2.4 cells + LPS group and DC2.4 cells + LPS + lin two groups are mature DCs morphology. Flow cytometry analysis confirmed that 500 ng / mL LPS can promote the expression of MHC class Ⅱ molecules, CD86 and CD80 on the surface of DC2.4 cells and enhance its stimulation of T cell proliferation and IFN-γ secretion. However, 500 ng / mL lin combined with 500 ng / mL LPS partially inhibited the immunoregulatory effect of DC2.4 cells. Conclusion: Lin can partially inhibit the immunomodulatory function of mature DC2.4 cells.