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目的 检测鼻NK/T细胞淋巴瘤中的穿孔素、颗粒酶B表达分布情况 ,为临床治疗和预后判断提供依据。方法 运用免疫组化S P法检测 11例鼻NK/T细胞淋巴瘤中穿孔素、颗粒酶B的表达和分布情况 ;同时与组织芯片中其他类型淋巴瘤进行比较研究 ,10例淋巴组织反应性增生作为对照。结果 11例鼻NK/T细胞淋巴瘤中均见穿孔素和颗粒酶B过度表达。以 6 0例淋巴瘤制成组织芯片的 4 2例B细胞淋巴瘤中见 11例少许穿孔素和 14例少许颗粒酶B阳性表达细胞散在分布于瘤细胞间 ,10例外周T细胞淋巴瘤中 8例少许表达穿孔素和 9例少许表达颗粒酶B。 2例间变性大细胞淋巴瘤见少许穿孔素和颗粒酶B阳性表达细胞。 2例霍奇金淋巴瘤阴性。NK/T细胞淋巴瘤的穿孔素和颗粒酶B表达程度与T、B淋巴瘤比较 ,差异有显著性 (P <0 0 1)。结论 穿孔素和颗粒酶B是鉴定活化的细胞毒性细胞的免疫标记物 ,可作为NK/T细胞淋巴瘤的诊断性标记物 ;其在外周T细胞淋巴瘤和B细胞淋巴瘤中的表达 ,反映了机体存在抗肿瘤的免疫反应机制。
Objective To detect the distribution of perforin and granzyme B in nasal NK / T cell lymphoma and to provide evidence for clinical treatment and prognosis. Methods Immunohistochemical SP method was used to detect the expression and distribution of perforin and granzyme B in 11 cases of nasal NK / T cell lymphoma. At the same time, compared with other types of lymphoma in tissue microarray, 10 cases of lymphoid tissue reactive hyperplasia as comparison. Results Perforin and granzyme B were overexpressed in 11 cases of nasal NK / T cell lymphoma. Of the 42 cases of B-cell lymphoma with 60 tissue-made lymphoma tissue samples, 11 cases of perforin and 14 cases of granzyme B-positive cells scattered in the tumor cells. Among 10 cases of peripheral T-cell lymphoma 8 cases expressed perforin and 9 cases expressed granzyme B little. 2 cases of anaplastic large cell lymphoma see a little perforin and granzyme B positive cells. Two cases of Hodgkin’s lymphoma were negative. The expression of perforin and granzyme B in NK / T cell lymphoma was significantly different from T and B lymphoma (P <0.01). CONCLUSION Perforin and granzyme B are immuno-markers that identify activated cytotoxic cells and serve as diagnostic markers of NK / T cell lymphomas; their expression in peripheral T-cell lymphoma and B-cell lymphoma reflects The body has anti-tumor immune response mechanism.