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目的研究全反式维A酸作用于人角质形成细胞HaCaT细胞后,通过分子水平检测Flg及Klf4的变化,以明确维A酸对皮肤屏障相关因子的影响及可能分子机制。方法 1μmol/L的全反式维A酸作用HaCaT 36h后,检测Flg,Klf4在基因及蛋白水平的变化。结果全反式维A酸作用HaCaT细胞36h后,实时定量PCR法及免疫印迹技术结果显示实验组Flg,Klf4表达下降。结论全反式维A酸作用于HaCaT细胞后,引起Flg及Klf4在基因及蛋白水平表达的下调,从分子生物学角度提示全反式维A酸可引起皮肤屏障的破坏。
Objective To investigate the effects of all-trans retinoic acid on human keratinocytes HaCaT cells and the changes of Flg and Klf4 at the molecular level to clarify the molecular mechanism of retinoic acid on the skin barrier-related factors. Methods After HaCaT treatment with 1 μmol / L all-trans retinoic acid for 36 h, the changes of Flg and Klf4 at gene and protein levels were examined. Results After all-trans retinoic acid treatment of HaCaT cells for 36h, real-time quantitative PCR and Western blotting showed that the expression of Flg and Klf4 in the experimental group decreased. Conclusion All-trans retinoic acid causes a down-regulation of the expression of Flg and Klf4 in HaCaT cells. It suggests that all-trans retinoic acid can cause skin barrier damage in molecular biology.