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[目的]探讨蝇蛆、茶叶、魔芋精粉、南瓜粉混合物对2型糖尿病大鼠免疫功能的影响。[方法]选择健康Wistar大鼠60只,将大鼠按随机数字表法分成2组:正常对照组16只,糖尿病造模组44只。糖尿病造模组大鼠以高脂高糖饲料诱导4周加小剂量链脲佐菌素(STZ)腹腔注射(30mg/kg)进行造模,空腹血糖≥16.7mmol/L为造模成功。将造模成功的糖尿病大鼠39只按随机数字表法分为2组:模型对照组(19只)和模型实验组(20只)。正常对照组和模型对照组饲以基础饲料;模型实验组饲以混合饲料。动物继续喂养8周后摘眼球取血,用全自动生化分析仪测定血糖、总蛋白(TP)、白蛋白(Alb)、免疫球蛋白IgG、IgM、补体C3、C4;用全自动血细胞分析仪测定白细胞、淋巴细胞;用全自动流式细胞仪测定CD3+,CD4+,CD8+。取动物的胸腺和脾脏组织,用分析天平称量并计算出脏器指数。[结果](1)注射STZ前,各组之间的血糖水平差异无统计学意义(P﹥0.05)。注射STZ1周后,糖尿病造模组的平均血糖值为(24.40±5.15)mmol/L,高于正常对照组,差异有统计学意义(P﹤0.05)。大鼠继续喂养8周后,模型对照组和模型实验组的血糖值高于正常对照组,差异有统计学意义(P﹤0.05);模型实验组的血糖值低于模型对照组,差异有统计学意义(P﹤0.05)。(2)模型对照组和模型实验组的总蛋白水平、IgG、C4、CD4+、胸腺指数均低于正常对照组,差异有统计学意义(P﹤0.05);模型对照组的白蛋白水平、IgM、淋巴细胞百分比、CD3+、CD4+/CD8+、脾指数均低于正常对照组,差异有统计学意义(P﹤0.05);模型实验组的总蛋白、白蛋白、IgG、IgM、淋巴细胞百分比、CD3+、CD4+、脾指数、胸腺指数均高于模型对照组,差异有统计学意义(P﹤0.05)。[结论](1)混合物对糖尿病大鼠有一定的降糖作用。(2)混合物可适当升高糖尿病大鼠的免疫水平。(3)蝇蛆、茶叶、魔芋精粉、南瓜粉混合物或许是2型糖尿病病人比较理想的增强免疫力的辅助食物。
[Objective] To investigate the effects of maggot, tea, konjac powder and pumpkin powder on immune function in type 2 diabetic rats. [Methods] Sixty healthy Wistar rats were selected and randomly divided into two groups according to random number table: 16 in normal control group and 44 in diabetic model group. Rats in diabetes model group were given intraperitoneal injection (30mg / kg) of streptozotocin (STZ) given high-fat and high-sugar diet for 4 weeks and fasting blood glucose≥16.7mmol / L was successfully established. Thirty-nine diabetic rats were divided into two groups according to random number table: model control group (n = 19) and model experimental group (n = 20). Normal control group and model control group were fed basal diet; model experimental group fed with mixed feed. Animals were continuously fed for 8 weeks and then blood was taken from the eyeball. Blood glucose, total protein (TP), albumin (Alb), immunoglobulin IgG, IgM and complement C3, C4 were measured by automatic biochemical analyzer. Determination of white blood cells, lymphocytes; using automated flow cytometry CD3 +, CD4 +, CD8 +. Thymus and spleen tissues of animals were weighed and weighed with an analytical balance and organ indicators calculated. [Results] (1) Before injection of STZ, there was no significant difference in blood glucose level among the groups (P> 0.05). After injecting STZ for 1 week, the mean blood glucose of diabetic model group was (24.40 ± 5.15) mmol / L, which was higher than that of normal control group, the difference was statistically significant (P <0.05). After 8 weeks of continuous feeding, the blood glucose of model control group and model experimental group were higher than that of normal control group (P <0.05); the blood glucose of model experimental group was lower than that of model control group Significance (P <0.05). (2) The levels of total protein, IgG, C4, CD4 + and thymus index in the model control group and the model experimental group were lower than those in the normal control group (P <0.05). The levels of albumin, IgM , The percentage of lymphocyte, CD3 +, CD4 + / CD8 + and spleen index were all lower than those in normal control group (P <0.05). The total protein, albumin, IgG, IgM, lymphocyte percentage, CD3 + , CD4 +, spleen index, thymus index were higher than the model control group, the difference was statistically significant (P <0.05). [Conclusion] (1) The mixture has a certain hypoglycemic effect on diabetic rats. (2) the mixture may be appropriate to raise the immune level of diabetic rats. (3) fly maggots, tea, konjac flour, pumpkin powder mixture may be the ideal type 2 diabetes patients to enhance the immunity of food supplement.