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目的：观察大鼠局灶性脑缺血再灌注后脑细胞凋亡率的时程变化。方法：插线法制备大 鼠大脑中动脉梗塞再灌注（ＭＣＡＯ／Ｒ）模型，用流式细胞仪检测缺血２ｈ再灌注６ｈ、１２ｈ、２４ｈ、４８ｈ、７２ｈ 的凋亡率。结果：缺血再灌注４８ｈ内，随再灌注时间的增加，脑细胞凋亡率逐渐升高，以再灌注４８ｈ凋 亡率最高（１３．１０％± １．３７％），明显高于再灌注６ｈ（３．３８％±０．７６％）， １２ｈ（６．９８％± １．６３％），与再灌 注 ２４ｈ（１１，６８％±１.４４％）无明显差别；灌注 ７２ｈ（１０．２０％±２．３０％）明显降低。结论：ＭＣＡＯ２ｈ再灌 注后，脑细胞凋亡的出现是一个动态过程，初期升高，２４ｈ～４８ｈ达到高峰，然后缓慢下降。
Objective: To observe the changes of apoptosis in brain cells after focal cerebral ischemia / reperfusion in rats. Methods: The middle cerebral artery occlusion and reperfusion (MCAO / R) model was established by the plug-in method. The apoptosis rate was detected by flow cytometry at 6h, 12h, 24h, 48h and 72h after reperfusion. Results: Within 48 hours after ischemia-reperfusion, the apoptosis rate of brain cells gradually increased with the increase of reperfusion time, and reached the peak at 48 hours after reperfusion (13.10% ± 1.37%), which was significantly higher than that of reperfusion 6h (3.38% ± 0.76%), 12h (6.98% ± 1.63%), no significant difference from reperfusion 24h (11.68% ± 1.44% 20% ± 2.30%) was significantly lower. Conclusion: After MCAO2h reperfusion, the appearance of apoptosis in brain cells is a dynamic process. The initial phase of MCAO2h apoptosis is peaked at 24h ~ 48h and then decreases slowly.